Practicals and demonstrations

ELECTROPHYSIOLOGY AND IMAGING

From the essentials to state of the art techniques for neurophysiologists

The course offers a choice of intensive, hands-on practicals and students attend numerous demonstrations of high-level techniques through visits to labs at the Université Paris Descartes and at the Ecole Normale Supérieure.

HANDS-ON

Three two-day blocks, which students choose from the following :

=> In 2018 we aim also to offer an electronics practical

Extracellular Field recording

In this practical you will learn to perform field recordings in hippocampal slices, induce and record LTP as well as using optical stimulation of channelrhodopsin-expressing inputs

Photolysis of caged neurotransmitters

Use a laser to trigger focal photorelease of L-glutamate, GABA in cerebellar slices. Learn how to calibrate photorelease like a pro, using caged fluorophores.

Optical bench - light sources, detectors and optical components

Use a dedicated optical bench to learn how to incorporate your own light sources, detectors and scanners into an existing setup.

Patch-clamp recording and synaptic stimulation in slices

Learn about slice preparation, recording, amplifier operation, optimisation of recordings and synaptic stimulation. Two setups are available.

Optical recording of Membrane potential with voltage sensitive membrane dyes

Learn how to optimise voltage-dye recordings using ultra-fast CCD or cMOS camera imaging, laser light sources and protocols for labelling neurons during whole-cell recording and imaging.

Imaging Calcium concentration in whole cell recording in slices

Learn how to use organic and genetically encoded calcium indicators, fast CCD imaging and LED illumination to record calcium responses from somata, dendrites and terminals during whole-cell recording.

DEMONSTRATIONS

Custom demonstrations of state of the art techniques

    • In vivo patch clamping
    • In vivo patch & calcium imaging
    • Super resolution microscopy
    • Holographic stimulation
    • Viral transfection strategies
    • Two-photon microscopes
    • Confocal microscopes
    • Light-sheet microscopes
    • 3D acousto-optic deflectors
    • And more…
INSTRUCTORS

Philippe Ascher

Paris Descartes

Jonathan Bradley

IBENS

Stéphane Dieudonné

IBENS

Michael Graupner

Paris Descartes

Boris Lamotte d'Incamps

Paris Descartes

David Ogden

Paris Descartes

Jorge Ramirez

Paris Descartes

Brandon Stell

Paris Descartes

Javier Zorrilla de San Martin

Paris Descartes

Céline Auger

Paris Descartes

Maia Brunstein

Paris Descartes

Desdemona Fricker

Paris Descartes

Marc Guillon

Paris Descartes

Joana Lourenço

ICM

Eirini Papagiakoumou

Paris Descartes

Nelson Rebola

ICM

German Sumbre

IBENS

Gary Bhumbra

University College London

Marco Canepari

Inserm Grenoble

Marta Gajowa

Paris Descartes

Carmel Howe

University of Nottingham

Benjamin Mathieu

IBENS

Rebecca Piskorowski

Noah Russell

University of Nottingham

Ludovic Tricoire

UPMC

Kris Blanchard

Paris Descartes

Igor Delvendahl

University of Zurich

Lyle Graham

Paris Descartes

Merouann Kassa

Paris Descartes

Vincenzo Marra

University of Leicester

Marko Popovic

Netherlands Institute for Neuroscience

Adrien Schramm

Paris Descartes/ICM

Federico Trigo

Paris Descartes