ELECTROPHYSIOLOGY AND IMAGING
From the essentials to state of the art techniques for neurophysiologists
The course offers a choice of intensive, hands-on practicals and students attend numerous demonstrations of high-level techniques through visits to labs at the Université Paris Descartes and at the Ecole Normale Supérieure.
HANDS-ON
Three two-day blocks, which students choose from the following :
Extracellular Field recording
In this practical you will learn to perform field recordings in hippocampal slices, induce and record LTP as well as using optical stimulation of channelrhodopsin-expressing inputs
Photolysis of caged neurotransmitters
Use a laser to trigger focal photorelease of L-glutamate, GABA in cerebellar slices. Learn how to calibrate photorelease like a pro, using caged fluorophores.
Optical bench - light sources, detectors and optical components
Use a dedicated optical bench to learn how to incorporate your own light sources, detectors and scanners into an existing setup.
Patch-clamp recording and synaptic stimulation in slices
Learn about slice preparation, recording, amplifier operation, optimisation of recordings and synaptic stimulation. Two setups are available.
Optical recording of Membrane potential with voltage sensitive membrane dyes
Learn how to optimise voltage-dye recordings using ultra-fast CCD or cMOS camera imaging, laser light sources and protocols for labelling neurons during whole-cell recording and imaging.
Imaging Calcium concentration in whole cell recording in slices
Learn how to use organic and genetically encoded calcium indicators, fast CCD imaging and LED illumination to record calcium responses from somata, dendrites and terminals during whole-cell recording.
ELECTRONIC BENCH
Learn the basics of analog and digital electronics. Essential knowledge for cellular electrophysiologists.
NEUROPIXEL
Discover high-density multielectrode recording and high-throughput analysis using Neuropixels.
DEMONSTRATIONS
Custom demonstrations of state of the art techniques
-
- In vivo patch clamping
- In vivo patch & calcium imaging
- Super resolution microscopy
- Holographic stimulation
- Viral transfection strategies
-
- Two-photon microscopes
- Confocal microscopes
- Light-sheet microscopes
- 3D acousto-optic deflectors
- And more…
INSTRUCTORS
Tim Harris
Janelia Farm, USA
Luiza Filipis
Inserm Grenoble
Samuel Garcia
CNRL
Louis Richevaux
Paris Descartes
Flavia Alusini
Paris Descartes
Maria Fernanda Niiño
Paris Descartes
David Ogden
Paris Descartes
Pierre Yger
Institut de la Vision
Brandon Stell
Paris Descartes
Maxime Beau
UCL, UK
Marin Manuel
Univ. Rhode Island, USA
Desdemona Fricker
Paris Descartes
Marco Diana
Paris Descartes
Joana Lourenço
ICM
Angela Vergnano
Paris Descartes
Nelson Rebola
ICM
German Sumbre
IBENS
Sébastien Wolf
IBENS
Marco Canepari
Inserm Grenoble
Mahesh Karnani
VU, The Netherlands
Boris Barbour
IBENS
Eric Schwartz
Paris Descartes
Benjamin Mathieu
IBENS
Antonin Singer
Paris Descartes
Ludovic Tricoire
IPS
Alan Montarras
IBENS
Laila Blömer
Inserm Grenoble
Igor Delvendahl
University of Zurich
Michael Graupner
Paris Descartes
Murat Orynbayev
Paris Descartes
Vincenzo Marra
University of Leicester
Nelson Rebola
ICM
Nicolas Gervasi
Collège de France
Federico Trigo
IIBCE, Uruguay