Practicals and demonstrations

ELECTROPHYSIOLOGY AND IMAGING

From the essentials to state of the art techniques for neurophysiologists

The course offers a choice of intensive, hands-on practicals and students attend numerous demonstrations of high-level techniques through visits to labs at the Université Paris Descartes and at the Ecole Normale Supérieure.

HANDS-ON

Three two-day blocks, which students choose from the following :

Extracellular Field recording

In this practical you will learn to perform field recordings in hippocampal slices, induce and record LTP as well as using optical stimulation of channelrhodopsin-expressing inputs

Photolysis of caged neurotransmitters

Use a laser to trigger focal photorelease of L-glutamate, GABA in cerebellar slices. Learn how to calibrate photorelease like a pro, using caged fluorophores.

Optical bench - light sources, detectors and optical components

Use a dedicated optical bench to learn how to incorporate your own light sources, detectors and scanners into an existing setup.

Patch-clamp recording and synaptic stimulation in slices

Learn about slice preparation, recording, amplifier operation, optimisation of recordings and synaptic stimulation. Two setups are available.

Optical recording of Membrane potential with voltage sensitive membrane dyes

Learn how to optimise voltage-dye recordings using ultra-fast CCD or cMOS camera imaging, laser light sources and protocols for labelling neurons during whole-cell recording and imaging.

Imaging Calcium concentration in whole cell recording in slices

Learn how to use organic and genetically encoded calcium indicators, fast CCD imaging and LED illumination to record calcium responses from somata, dendrites and terminals during whole-cell recording.

ELECTRONIC BENCH

Learn the basics of analog and digital electronics. Essential knowledge for cellular electrophysiologists.

NEUROPIXEL

Discover high-density multielectrode recording and high-throughput analysis using Neuropixels.

DEMONSTRATIONS

Custom demonstrations of state of the art techniques

    • In vivo patch clamping
    • In vivo patch & calcium imaging
    • Super resolution microscopy
    • Holographic stimulation
    • Viral transfection strategies
    • Two-photon microscopes
    • Confocal microscopes
    • Light-sheet microscopes
    • 3D acousto-optic deflectors
    • And more…
INSTRUCTORS

Tim Harris

Janelia Farm, USA

Luiza Filipis

Inserm Grenoble

Samuel Garcia

CNRL

Louis Richevaux

Paris Descartes

Flavia Alusini

Paris Descartes

Maria Fernanda Niiño

Paris Descartes

David Ogden

Paris Descartes

Pierre Yger

Institut de la Vision

Brandon Stell

Paris Descartes

Maxime Beau

UCL, UK

Marin Manuel

Univ. Rhode Island, USA

Desdemona Fricker

Paris Descartes

Marco Diana

Paris Descartes

Joana Lourenço

ICM

Angela Vergnano

Paris Descartes

Nelson Rebola

ICM

German Sumbre

IBENS

Sébastien Wolf

IBENS

Marco Canepari

Inserm Grenoble

Mahesh Karnani

VU, The Netherlands

Boris Barbour

IBENS

Eric Schwartz

Paris Descartes

Benjamin Mathieu

IBENS

Antonin Singer

Paris Descartes

Ludovic Tricoire

IPS

Alan Montarras

IBENS

Laila Blömer

Inserm Grenoble

Igor Delvendahl

University of Zurich

Michael Graupner

Paris Descartes

Murat Orynbayev

Paris Descartes

Vincenzo Marra

University of Leicester

Nelson Rebola

ICM

Nicolas Gervasi

Collège de France

Federico Trigo

IIBCE, Uruguay