ELECTROPHYSIOLOGY AND IMAGING
From the essentials to state of the art techniques for neurophysiologists
The course offers a choice of intensive, hands-on practicals and students attend numerous demonstrations of high-level techniques through visits to labs at the Université Paris Cité, the Ecole Normale Supérieure, the Paris Brain Institute (ICM) and the Institut de la Vision.
Consolidate your knowledge of the concepts of electronics and optics with the two “benches”. Everybody does these.
Intensive training on basic concepts
Build operational-amplifier circuits, learn about digital electronics, understand what your interface does.
Learn about what is inside a microscope and how to couple exetrnal optics to it.
Three two-day blocks, which students choose from the following :
Mandatory Patch training
To unlock advances patch workshops an intitial two days traning is provided to teach students to do their own DIC in vitro patching.
Photolysis of caged neurotransmitters
Use a laser to trigger focal photorelease of L-glutamate, GABA in cerebellar slices. Learn how to calibrate photorelease like a pro, using caged fluorophores.
Extracellular Field recording
In this practical you will learn to perform field recordings in hippocampal slices, induce and record LTP as well as using optical stimulation of channelrhodopsin-expressing inputs
Patch-clamp recording and synaptic stimulation in slices
Learn about slice preparation, recording, amplifier operation, optimisation of recordings and synaptic stimulation. Two setups are available.
Sodium imaging with ultra-fast cameras
Learn how to use ultra-fast CCD or cMOS camera imaging and laser light sources to image neuronal signals in slices; the practical will apply these techniques to imaging intracellular sodium ions.
Imaging Calcium concentration in whole cell recording in slices
Learn how to use organic and genetically encoded calcium indicators, fast CCD imaging and LED illumination to record calcium responses from somata, dendrites and terminals during whole-cell recording.
2-photon calcium imaging
Learn to use 2-photon microscopy to measure intracellular calcium concentrations in subcellular compartments.
Discover high-density multielectrode recording and high-throughput analysis using Neuropixels.
2-photon voltage imaging
Discover the latest techniques for voltage recordings using high-speed 2-photon imaging.
Learn how to assemble and deploy open-source miniscopes to perform in vivo calcium recordings in freely-moving animals.
Custom demonstrations of state of the art techniques
- Holographic stimulation
- Tissue Clearing
- Light-sheet microscopes
- 3D acousto-optic deflectors
- And more…
Janelia Farm, USA
Maria Fernanda Niiño
Institut de la Vision
Univ. Rhode Island, USA
VU, The Netherlands
University of Zurich
University of Leicester
Collège de France